A Bioorthogonal Probe for Multiscale Imaging by 19F-MRI and Raman Microscopy: From Whole Body to Single Cells
Cristina Chirizzi, Francesca Baldelli Bombelli and Pierangelo Metrangolo have recently published an article on JACS about "A Bioorthogonal Probe for Multiscale Imaging by 19F-MRI and Raman Microscopy: From Whole Body to Single Cells".
The full paper can be found at the following link: A Bioorthogonal Probe for Multiscale Imaging by 19F-MRI and Raman Microscopy: From Whole Body to Single Cells.
Abstract. Molecular imaging techniques are essential tools for better investigating biological processes and detecting disease biomarkers with improvement of both diagnosis and therapy monitoring. Often, a single imaging technique is not sufficient to obtain comprehensive information at different levels. Multimodal diagnostic probes are key tools to enable imaging across multiple scales. The direct registration of in vivo imaging markers with ex vivo imaging at the cellular level with a single probe is still challenging. Fluorinated (19F) probes have been increasingly showing promising potentialities for in vivo cell tracking by 19F-MRI. Here we present the unique features of a bioorthogonal 19F-probe that enables direct signal correlation of MRI with Raman imaging. In particular, we reveal the ability of PERFECTA, a superfluorinated molecule, to exhibit a remarkable intense Raman signal distinct from cell and tissue fingerprints. Therefore, PERFECTA combines in a single molecule excellent characteristics for both macroscopic in vivo19F-MRI, across the whole body, and microscopic imaging at tissue and cellular levels by Raman imaging.
How to cite the article:
Cristina Chirizzi, Carlo Morasso, Alessandro Aldo Caldarone, Matteo Tommasini, Fabio Corsi, Linda Chaabane, Renzo Vanna, Francesca Baldelli Bombelli, and Pierangelo Metrangolo, A Bioorthogonal Probe for Multiscale Imaging by 19F-MRI and Raman Microscopy: From Whole Body to Single Cells, J. Am. Chem. Soc. 2021, 143, 31, 12253–12260
DOI: https://doi.org/10.1021/jacs.1c05250
