Superfluorinated Extracellular Vesicles for In Vivo Imaging by ¹⁹F-MRI

Nazeeha Ayaz, Dr. Cristina Chirizzi, Prof. Pierangelo Metrangolo, Prof. Francesca Baldelli Bombelli and others have recently published an article on ACS Applied Materials & Interfaces about "Superfluorinated Extracellular Vesicles for In Vivo Imaging by 19F-MRI".

The full paper can be found at the following link: Superfluorinated Extracellular Vesicles for In Vivo Imaging by 19F-MRI.

Abstract. Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and have great potential as efficient delivery vectors. However, a better understanding of EV in vivo behavior is hampered by the limitations of current imaging tools. In addition, chemical labels present the risk of altering the EV membrane features and, thus, in vivo behavior. 19F-MRI is a safe bioimaging technique providing selective images of exogenous probes. Here, we present the first example of fluorinated EVs containing PERFECTA, a branched molecule with 36 magnetically equivalent 19F atoms. A PERFECTA emulsion is given to the cells, and PERFECTA-containing EVs are naturally produced. PERFECTA-EVs maintain the physicochemical features, morphology, and biological fingerprint as native EVs but exhibit an intense 19F-NMR signal and excellent 19F relaxation times. In vivo 19F-MRI and tumor-targeting capabilities of stem cell-derived PERFECTA-EVs are also proved. We propose PERFECTA-EVs as promising biohybrids for imaging biodistribution and delivery of EVs throughout the body.


How to cite:
María Sancho-Albero, Nazeeha Ayaz, Victor Sebastian, Cristina Chirizzi, Miguel Encinas-Gimenez, Giulia Neri, Linda Chaabane, Lluís Luján, Pilar Martin-Duque, Pierangelo Metrangolo, Jesús Santamaría, and Francesca Baldelli Bombelli Superfluorinated Extracellular Vesicles for In Vivo Imaging by 19F-MRI, ACS Appl. Mater. Interfaces, 2023, TBD



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