Pivotal role of the protein corona in the cell uptake of fluorinated nanoparticles with increased sensitivity for ¹⁹F-MR imaging

Lodovico Gatti, Dr. Cristina Chirizzi, Pietro Milesi, Prof. Pierangelo Metrangolo, Prof. Francesca Baldelli Bombelli and others have recently published an article on Nanoscale Advances about "Pivotal role of the protein corona in the cell uptake of fluorinated nanoparticles with increased sensitivity for ¹⁹F-MR imaging".

The full paper can be found at the following link: Pivotal role of the protein corona in the cell uptake of fluorinated nanoparticles with increased sensitivity for ¹⁹F-MR imaging.

Abstract. In vivo cell tracking by non-invasive imaging technologies is needed to accelerate the clinical translation of innovative cell-based therapies. In this regard, ¹⁹F-MRI has recently gained increased attention for unbiased localization of labeled cells over time. To push forward the use of ¹⁹F-MRI for cell tracking, the development of highly performant ¹⁹F-probes is required. PLGA-based NPs containing PERFECTA, a multibranched superfluorinated molecule with an optimal MRI profile thanks to its 36 magnetically equivalent fluorine atoms, are promising ¹⁹F-MRI probes. In this work we demonstrate the importance of the surface functionalization of these NPs in relation to their interaction with the biological environment, stressing the pivotal role of the formation of the protein corona (PC) in their cellular labelling efficacy. In particular, our studies showed that the formation of PC NPs strongly promotes the cellular internalization of these NPs in microglia cells. We advocate that the formation of PC NPs in the culture medium can be a key element to be used for the optimization of cell labelling with a considerable increase of the detection sensitivity by 19F-MRI.

 

How to cite:
Lodovico Gatti, Cristina Chirizzi, Giulia Rotta, Pietro Milesi, María Sancho-Albero, Victor Sebastián, Anna Mondino, Jesús Santamaría, Pierangelo Metrangolo, Linda Chaabane and Francesca Baldelli Bombelli Pivotal role of the protein corona in the cell uptake of fluorinated nanoparticles with increased sensitivity for ¹⁹F-MR imaging, Nanoscale Adv., 2023, 5, 3749-3760
DOI: https://doi.org/10.1039/D3NA00229B